NEETZoologyBiotechnology - Principles and Processes
When isolating the pure DNA from a bacterial cell, the cell should be treated with
Options
- ALysozyme
- BCellulase
- CChitanase
- DDeoxyribonuclease
Correct answer
A. Lysozyme
Step-by-step solution
Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). You know that genes are located on long molecules of DNA intertwined with proteins such as histones