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After 30 cycles of PCR, how many times will DNA be amplified?

Options

  1. AThousand times
  2. BMillion times
  3. CBillion times
  4. DHundreds times

Correct answer

C. Billion times

Step-by-step solution

The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion times, i.e., 1 billion copies are made. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation of double stranded DNA.

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