NEETZoologyBiotechnology - Principles and Processes
Read the following Assertion and Reason and choose the correct option. Assertion: The matrix used in gel electrophoresis should have controllable pore size. Reason: Agarose concentration can be changed to change pore sizes.
Options
- ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
- BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
- CAssertion is true but the Reason is false
- DBoth Assertion and Reason are false
Correct answer
A. Both Assertion and Reason are true and Reason is correct explanation of Assertion
Step-by-step solution
Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.