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Read the following Assertion and Reason and choose the correct option. Assertion: E.coli having pBR322 with DNA insert at BamH | site cannot grow in medium containing tetracycline. Reason: Recognition site for BamH | is present in tetR region of pBR322.

Options

  1. ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
  2. BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
  3. CAssertion is true but the Reason is false
  4. DBoth Assertion and Reason are false

Correct answer

A. Both Assertion and Reason are true and Reason is correct explanation of Assertion

Step-by-step solution

The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322.

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