NEETZoologyBiotechnology - Principles and Processes
Read the following Assertion and Reason and choose the correct option. Assertion: Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin
Options
- ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
- BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
- CAssertion is true but the Reason is false
- DBoth Assertion and Reason are false
Correct answer
A. Both Assertion and Reason are true and Reason is correct explanation of Assertion
Step-by-step solution
Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). You know that genes are located on long molecules of DNA interwined with proteins such as histones.