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NEETZoologyBiotechnology - Principles and Processes

Read the following Assertion and Reason and choose the correct option. Assertion: PCR primers must not have self complementary regions. Reason: Self complementary regions result in hairpin structures adversely affecting the PCR.

Options

  1. ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
  2. BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
  3. CAssertion is true but the Reason is false
  4. DBoth Assertion and Reason are false

Correct answer

A. Both Assertion and Reason are true and Reason is correct explanation of Assertion

Step-by-step solution

PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template.

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