NEETZoologyBiotechnology - Principles and Processes
Read the following Assertion and Reason and choose the correct option. Assertion: Restriction digestion is a process of cutting DNA by restriction enzyme. Reason: DNA ligase joins two DNAs.
Options
- ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
- BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
- CAssertion is true but the Reason is false
- DBoth Assertion and Reason are false
Correct answer
B. Both Assertion and Reason are true but the Reason is not the correct explanation of Assertion
Step-by-step solution
The cutting of DNA at specific locations became possible with the discovery of the so-called molecular scissors’– restriction enzymes. The cut piece of DNA was then linked with the plasmid DNA. These plasmid DNA act as vectors to transfer the piece of DNA attached to it. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends.