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Read the following Assertion and Reason and choose the correct option. Assertion: During gel electrophoresis, the DNA fragments move towards the anode Reason: DNA fragments are negatively charged molecules

Options

  1. ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
  2. BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
  3. CAssertion is true but the Reason is false
  4. DBoth Assertion and Reason are false

Correct answer

A. Both Assertion and Reason are true and Reason is correct explanation of Assertion

Step-by-step solution

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provided

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