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Read the following Assertion and Reason and choose the correct option. Assertion: Taq Polymerase is involved in PCR technique Reason: This enzyme remains active during high temperature, including denaturation of double stranded DNA

Options

  1. ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
  2. BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
  3. CAssertion is true but the Reason is false
  4. DBoth Assertion and Reason are false

Correct answer

A. Both Assertion and Reason are true and Reason is correct explanation of Assertion

Step-by-step solution

Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation of double stranded DNA. The amplified fragment if desired can now be used to ligate with a vector for further cloning

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