NEETZoologyBiotechnology - Principles and Processes
Read the following Assertion and Reason and choose the correct option. Assertion: During transformation, in order to force bacteria to take up the plasmid, the bacterial cell must first be made 'competent' to take up DNA Reason: This is done by treating them with a specific concentration of a monovalent cation such as sodim
Options
- ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
- BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
- CAssertion is true but the Reason is false
- DBoth Assertion and Reason are false
Correct answer
C. Assertion is true but the Reason is false
Step-by-step solution
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes. Why? In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium