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Read the following Assertion and Reason and choose the correct option. Assertion: If one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number Reason: Ori is responsible for controlling the copy number of the linked DNA/target DNA

Options

  1. ABoth Assertion and Reason are true and Reason is correct explanation of Assertion
  2. BBoth Assertion and Reason are true but the Reason is not the correct explanation of Assertion
  3. CAssertion is true but the Reason is false
  4. DBoth Assertion and Reason are false

Correct answer

A. Both Assertion and Reason are true and Reason is correct explanation of Assertion

Step-by-step solution

plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA. Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Some plasmids may have only one or two copies per cell whereas others may have 15-100 copies per cell. Their numbers can go even higher. If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its numbers equal to the copy num

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