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NEETZoologyBiotechnology - Principles and Processes

Read the following statements and choose the correct option: 1. Taq polymerase extends the primers using the nucleotides provided in the reaction 2. In genetic engineering, antibiotics are used as sequences from where replication starts 3. DNA fragments are separated according to their charge only, in agarose gel elctrophoresis 4. Transformation is a procedure through which a piece of DNA is integrated into the genom

Options

  1. A2 and 3 are incorrect
  2. BAll are correct
  3. C3 and 5 are incorrect
  4. D1 and 2 are correct

Correct answer

A. 2 and 3 are incorrect

Step-by-step solution

Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation of double stranded DNA. The amplified fragment if desired can now be used to ligate with a vector for further cloning

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