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NEETZoologyBiotechnology - Principles and Processes

Read the following statements and choose the correct option: 1. When the transformed cells on agar plates containing ampicillin are spread, both transformed and non transformed cells will grow 2. Restriction enzymes are used in isolation and separation of DNA from other macromolecules 3. Downstream processing is one of the steps of rDNA technology 4.Disarmed pathogen vectors are also used in transfer of rDNA into the

Options

  1. A2 and 3 are correct
  2. B1, 2 and 3 are incorrect
  3. C1 and 2 are incorrect
  4. DOnly 4 is correct

Correct answer

C. 1 and 2 are incorrect

Step-by-step solution

Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).

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