NEETZoologyBiotechnology - Principles and Processes
Read the following statements and choose the correct option: 1. The separated DNA fragments in Gel Electrophoresis can be visualised only after staining the DNA with a compound known as ethidium chloride 2. One cannot see pure DNA fragments in the visible light and without staining 3. You can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light 4. The separated bands of DNA are c
Options
- ABoth 2 and 4 are correct
- BOnly 1 and 4 are incorrect
- CAll are incorrect
- DBoth 1 and 2 are incorrect
Correct answer
A. Both 2 and 4 are correct
Step-by-step solution
The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light (Figure 9.3). The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used