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Read the following statements and choose the correct option: 1. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning 2. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. 3. The recombinant plasmids will lose antibiotic resistance due to insertion of foreign DNA but can s

Options

  1. AOnly 4 is incorrect
  2. BOnly 3 is incorrect
  3. CBoth 1 and 2 are correct
  4. DAll are correct

Correct answer

B. Only 3 is incorrect

Step-by-step solution

Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinant ones by pl

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