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Read the following statements and choose the correct option: 1. Selection of recombinants due to inactivation of antibiotics is an easy procedure because it requires simultaneous plating on two plates having different antibiotics. 2. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. 3. Presence of insert results into insertional inactivation o

Options

  1. AOnly 2 is correct
  2. BOnly 1 and 2 are correct
  3. CBoth 2 and 3 are correct
  4. DBoth 1 and 3 are incorrect

Correct answer

C. Both 2 and 3 are correct

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gene

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