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NEETZoologyBiotechnology - Principles and Processes

Read the following statements and choose the correct option: 1. Since DNA is a hydrophobic molecule, it cannot pass through cell membranes. 2. Host is made competent by treating them with a specific concentration of a monovalent cation, such as sodium 3. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them 420C (heat shock) at long 4. In orde

Options

  1. AOnly 4 is correct
  2. BBoth 1 and 4 are correct
  3. COnly 2 is incorrect
  4. DOnly 3 is incorrect

Correct answer

A. Only 4 is correct

Step-by-step solution

Since DNA is a hydrophilic molecule, it cannot pass through cell membranes. Why? In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall.

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