NEETZoologyBiotechnology - Principles and Processes
Read the following statements and choose the correct option: 1. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme. 2. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion. 3. DNA is a negatively charged molecule, hence it moves towards the positive electrode (ano
Options
- AOnly 5 is incorrect
- BBoth 1 and 5 are correct
- CBoth 2 and 4 re correct
- DOnly 5 is correct
Correct answer
A. Only 5 is incorrect
Step-by-step solution
Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion. DNA is a negatively charged molecule, hence it moves towards the positive electrode (anode) (Figure 9.3). The process is repeated with the vector DNA also.