NEETZoologyBiotechnology - Principles and Processes
Read the following statements and choose the correct option: 1. In PCR, multiple copies of the gene (or DNA) of interest is synthesised in vitro using four sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. The enzyme extends the primers using the nucleotides provided in the reaction and the vector DNA as template 3. Such repe
Options
- AAll are incorrect
- BBoth 1 and 2 are correct
- COnly 3 is incorrect
- DBoth 3 and 4 are correct
Correct answer
A. All are incorrect
Step-by-step solution
PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template.