NEETZoologyBiotechnology - Principles and Processes
Read the following statements and choose the correct option: 1. When you insert a piece of alien DNA into a cloning vector and transfer it into a bacterial, plant or animal cell, the alien DNA gets multiplied. 2. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale 3. If any protein encoding gene
Options
- ABoth 1 and 2 are incorrect
- BOnly 4 is incorrect
- COny 2 is correct
- DAll are incorrect
Correct answer
B. Only 4 is incorrect
Step-by-step solution
After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale. Can you think of any reason why there is a need for large-scale production? If any protein encoding gene is expressed in a heterologous host, it is called a recombinant protein. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. The cultures may be used for extracting the desired protein and then pu