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Insertional Inactivation Examples A. b-Galactosidase Gene 1. Inactivation leads to colorless colonies B. Tetracycline Resistance Gene 2. Used to select recombinants C. Chromogenic Substrate 3. Gene inactivated by the insertion of foreign DNA D. Ampicillin Resistance Gene 4. Remains functional even in recombinants

Options

  1. AA - 1, B - 2, C - 3, D - 4
  2. BA - 1, B - 3, C - 2, D - 4
  3. CA - 4, B - 3, C - 1, D - 2
  4. DA - 1, B - 4, C - 2, D - 3

Correct answer

B. A - 1, B - 3, C - 2, D - 4

Step-by-step solution

The b-galactosidase gene's inactivation leads to colorless colonies, the tetracycline resistance gene can be inactivated by the insertion of foreign DNA, the chromogenic substrate is used to select recombinants, and the ampicillin resistance gene remains functional even in recombinants.

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