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NEETZoologyBiotechnology - Principles and Processes

DNA Entry into Bacteria Process Description A. Competent Cells 1. Increases DNA entry through pores in cell walls B. Calcium Treatment 2. Brief exposure to 42°C C. Heat Shock 3. Cooling step before heat shock D. Ice Incubation 4. Cells ready to take up DNA

Options

  1. AA - 4, B - 1, C - 2, D - 3
  2. BA - 1, B - 4, C - 2, D - 3
  3. CA - 3, B - 2, C - 4, D - 1
  4. DA - 2, B - 1, C - 3, D - 4

Correct answer

A. A - 4, B - 1, C - 2, D - 3

Step-by-step solution

Competent cells are those ready to take up DNA, calcium treatment increases the efficiency of DNA entry, heat shock involves brief exposure to 42°C, and ice incubation is used to prepare cells before heat shock

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