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The role of DNA ligase in the construction of a recombinant DNA molecule is:

Options

  1. AFormation of a phosphodiester bond between two DNA fragments
  2. BFormation of hydrogen bonds between sticky ends of DNA fragments
  3. CLigation of all purine and pyrimidine bases
  4. DNone of the above

Correct answer

A. Formation of a phosphodiester bond between two DNA fragments

Step-by-step solution

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases.

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