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Regarding the restriction endonuclease Hind II, what does the Roman numeral 'II' indicate, and what is the length of its specific recognition sequence?

Options

  1. AOrder of discovery of the bacterial strain ; 6 base pairs
  2. BOrder of isolation of the enzyme from the bacterial strain ; 4 base pairs
  3. COrder of isolation of the enzyme from the bacterial strain ; 6 base pairs
  4. DThe species of the bacteria ; 8 base pairs

Correct answer

C. Order of isolation of the enzyme from the bacterial strain ; 6 base pairs

Step-by-step solution

According to the nomenclature rules for restriction enzymes, the Roman numeral following the name indicates the order in which the enzyme was isolated from that strain of bacteria. Hind II was the first restriction endonuclease to be isolated, and it always cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs. Option 1 is incorrect because the Roman numeral does not indicate the order of discovery of the bacterial strain. Option 2 is incorrect because the recognition sequenc

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