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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In order to link alien DNA, a cloning vector needs to have very few, preferably single, recognition sites for commonly used restriction enzymes. Reason (R): Presence of more than one recognition site for a restriction enzyme within the vector will generate several fragments, which will complicate t

Options

  1. ABoth Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A)
  2. BBoth Assertion (A) and Reason (R) are true but Reason (R) is not the correct explanation of Assertion (A)
  3. CAssertion (A) is true but Reason (R) is false
  4. DAssertion (A) is false but Reason (R) is true

Correct answer

A. Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A)

Step-by-step solution

Assertion (A) is true. For a cloning vector to be ideal, it should have a single recognition site for the commonly used restriction enzymes to allow the insertion of alien DNA at a specific location without fragmenting the vector. Reason (R) is also true and correctly explains the assertion. If a vector has multiple recognition sites for the same restriction enzyme, the enzyme will cut the vector at all those sites. This generates several DNA fragments, which makes the ligation of the alien DNA and the overall gene

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