How many of the following statements are correct regarding the formation of recombinant DNA? (A) Unless vector and source DNA are cut with the same restriction enzyme, the recombinant vector molecule cannot be created. (B) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites to leave single-stranded overhangs. (C) The sticky ends form hydrogen bonds with their complementary

Step-by-step solution

Statement (A) is correct: Using the same restriction enzyme ensures that both the vector and the source DNA have complementary sticky ends, which is essential for creating a recombinant molecule. Statement (B) is correct: Restriction enzymes like EcoRI cut the DNA slightly away from the centre of the palindromic recognition sequence, but between the same two bases on opposite strands, leaving single-stranded overhangs. Statement (C) is correct: The single-stranded overhanging regions are called sticky ends because