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If a polymerase chain reaction (PCR) is performed for 30 cycles, approximately how many times will the target DNA segment be amplified?

Options

  1. A60 times
  2. B1 million times
  3. C1 trillion times
  4. D1 billion times

Correct answer

D. 1 billion times

Step-by-step solution

According to NCERT, in a polymerase chain reaction (PCR), the DNA segment is amplified approximately 1 billion times if the process of replication of DNA is repeated 30 times. This is because the amplification follows an exponential growth pattern of 2^n , where n is the number of cycles ( 2³⁰ 10^9 ). Using a linear calculation like 30 2 = 60 is incorrect. Answer: 1 billion times

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