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During a gel electrophoresis experiment, a student observes characteristic bright orange fluorescent bands of separated DNA fragments. Which of the following specific combinations of stain and radiation must have been used by the student to achieve this visualisation?

Options

  1. AEthidium bromide and visible light
  2. BMethylene blue and UV radiation
  3. CEthidium bromide and infrared radiation
  4. DEthidium bromide and UV radiation

Correct answer

D. Ethidium bromide and UV radiation

Step-by-step solution

The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to ultraviolet (UV) radiation. Pure DNA fragments cannot be seen under visible light or infrared radiation. Other stains like methylene blue do not produce the characteristic bright orange fluorescent bands specific to the ethidium bromide and UV radiation combination. Answer: Ethidium bromide and UV radiation

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