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Read the following statements regarding the tools of recombinant DNA technology: (I) Unless the vector and source DNA are cut with the same restriction enzyme, the recombinant vector molecule cannot be created. (II) Exonucleases are primarily used to create sticky ends in cloning vectors. (III) The sticky ends form hydrogen bonds with their complementary cut counterparts without the help of DNA ligase. (IV) DNA ligas

Options

  1. AOnly one
  2. BOnly two
  3. COnly three
  4. DAll four

Correct answer

C. Only three

Step-by-step solution

Statement (I) is correct. Cutting both the vector and the source DNA with the same restriction enzyme ensures that they have compatible sticky ends, which is essential for creating a recombinant vector molecule. Statement (II) is incorrect. Exonucleases remove nucleotides from the ends of the DNA. It is restriction endonucleases that make cuts at specific positions within the DNA to create sticky ends. Statement (III) is correct. Sticky ends spontaneously form hydrogen bonds with their complementary counterparts du

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