Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

A researcher intends to achieve gene cloning by obtaining multiple identical copies of an alien DNA molecule inside a bacterial host. To initiate the replication of this alien DNA, it must be linked to which of the following specific sequences in the cloning vector?

Options

  1. AOrigin of replication (ori)
  2. BSelectable marker
  3. CPromoter sequence
  4. DRecognition site

Correct answer

A. Origin of replication (ori)

Step-by-step solution

Gene cloning involves obtaining multiple identical copies of a particular DNA molecule. For an alien piece of DNA to replicate and multiply inside a host cell, it must be linked to a specific DNA sequence responsible for controlling and initiating replication. This sequence is known as the origin of replication (ori). A selectable marker is used to identify and eliminate non-transformants, not to start replication. A promoter sequence is required for the transcription of a gene into RNA. A recognition site is a spe

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs