Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

A student is trying to isolate pure DNA from a fungal cell. The student is provided with the following list of enzymes: A. Chitinase B. Cellulase C. Ribonuclease D. Protease E. Lysozyme Which combination of enzymes is essential to obtain pure DNA from the fungal cell?

Options

  1. AA, B and C only
  2. BB, C and D only
  3. CA, C and E only
  4. DA, C and D only

Correct answer

D. A, C and D only

Step-by-step solution

To isolate DNA from a fungal cell, the cell wall must first be broken down. The fungal cell wall is composed of chitin, which is degraded by the enzyme chitinase. Cellulase is used to break down plant cell walls, and lysozyme is used for bacterial cell walls. Once the cell is broken open, macromolecules such as RNA and proteins must be removed to obtain pure DNA. RNA is degraded by ribonuclease, and proteins are degraded by protease. Therefore, the essential enzymes for isolating pure DNA from a fungal cell are chi

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs