Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

An enzyme isolated from a bacterium found in hot springs is widely used in the Polymerase Chain Reaction (PCR). Identify the source bacterium, the name of the enzyme, and the specific step of the PCR cycle (along with its approximate temperature) where this enzyme actively synthesizes DNA.

Options

  1. AThermus aquaticus, Taq polymerase, Denaturation at 94^ C
  2. BAgrobacterium tumefaciens, DNA ligase, Annealing at 52^ C
  3. CThermus aquaticus, Taq polymerase, Extension at 72^ C
  4. DThermus aquaticus, Taq polymerase, Annealing at 52^ C

Correct answer

C. Thermus aquaticus, Taq polymerase, Extension at 72^ C

Step-by-step solution

The thermostable enzyme Taq polymerase is isolated from the thermophilic bacterium Thermus aquaticus, which naturally inhabits hot springs. In the Polymerase Chain Reaction (PCR), the cycle consists of three main steps: Denaturation (around 94^ C ), Annealing (around 52^ C ), and Extension (around 72^ C ). While Taq polymerase survives the high temperature of the denaturation step, it actively catalyzes the polymerization of nucleotides (synthesizes DNA) during the extension step at approximately 72^ C . Answer: Th

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs