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Match List I with List II regarding gel electrophoresis: List I List II A. Agarose I. Extraction of separated DNA bands from the gel piece B. Ethidium bromide II. Positive electrode towards which DNA fragments move C. Elution III. Natural polymer providing the sieving effect D. Anode IV. Compound used to stain DNA for visualization under UV light Choose the correct answer from the options given below:

Options

  1. AA-III, B-I, C-IV, D-II
  2. BA-III, B-IV, C-I, D-II
  3. CA-I, B-IV, C-III, D-II
  4. DA-III, B-II, C-I, D-IV

Correct answer

B. A-III, B-IV, C-I, D-II

Step-by-step solution

Agarose is a natural polymer extracted from seaweeds that provides the sieving effect for the separation of DNA fragments. Ethidium bromide is a compound used to stain the separated DNA fragments, which can then be visualized as bright orange bands under UV light. Elution is the process where the separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. The anode is the positive electrode. Since DNA is negatively charged, it moves towards the anode during gel electrophoresis. Answer:

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