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Match List-I with List-II regarding the isolation of genetic material: List-I List-II (A) Plant cells (I) Chitinase (B) Fungal cells (II) Ribonuclease (C) RNA (III) Protease (D) Proteins (IV) Cellulase Choose the correct answer from the options given below:

Options

  1. A(A)-(I), (B)-(IV), (C)-(II), (D)-(III)
  2. B(A)-(IV), (B)-(I), (C)-(III), (D)-(II)
  3. C(A)-(IV), (B)-(I), (C)-(II), (D)-(III)
  4. D(A)-(IV), (B)-(II), (C)-(I), (D)-(III)

Correct answer

C. (A)-(IV), (B)-(I), (C)-(II), (D)-(III)

Step-by-step solution

In order to isolate DNA, the cells must be broken open. This is achieved by treating the cells with specific enzymes. Plant cells are treated with cellulase to degrade the cellulosic cell wall, while fungal cells are treated with chitinase to degrade the chitinous cell wall. Once the cells are lysed, other macromolecules need to be removed. RNA is removed by treatment with ribonuclease, whereas proteins are removed by treatment with protease. This ensures that the purified DNA can be precipitated out later. Answer:

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