A specific restriction endonuclease is observed to produce DNA fragments with single-stranded overhanging stretches, commonly known as sticky ends. To generate such ends, what must be true about the specific location where this enzyme cuts the DNA double helix?

Step-by-step solution

Restriction enzymes can produce either blunt ends or sticky ends depending on where they cut the DNA. To produce sticky ends (single-stranded overhanging stretches), the enzyme must cut the DNA a little away from the centre of the palindrome sites, but strictly between the same two bases on the opposite strands. This staggered cut leaves single-stranded portions at the ends. Cutting exactly at the centre of the palindromic site (Option 1) produces blunt ends. Restriction endonucleases typically cut between the same