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A specific restriction endonuclease recognises the following palindromic nucleotide sequence in DNA and cleaves it: 5' G A A T T C 3' 3' C T T A A G 5' Identify the specific biological source from which this restriction enzyme is isolated.

Options

  1. AHaemophilus influenzae Rd
  2. BBacillus amyloliquefaciens H
  3. CEscherichia coli RY 13
  4. DEscherichia coli R 245

Correct answer

C. Escherichia coli RY 13

Step-by-step solution

The given palindromic nucleotide sequence ( 5' G A A T T C 3' ) is specifically recognised and cleaved by the restriction endonuclease EcoRI. According to the conventions for naming restriction enzymes, the first letter comes from the genus and the second two letters come from the species of the prokaryotic cell from which they were isolated. For EcoRI, it is isolated from the bacterium Escherichia coli strain RY 13. Haemophilus influenzae Rd is the source of HindIII, and Bacillus amyloliquefaciens H is the source

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