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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Insertion of an alien DNA at the Pvu I site of pBR322 leads to susceptibility of the transformants to ampicillin. Reason (R): The Pvu I site is located within the 'rop' gene, which codes for proteins involved in the replication of the plasmid. In the light of the above statements, choose the most a

Options

  1. ABoth (A) and (R) are correct and (R) is the correct explanation of (A)
  2. BBoth (A) and (R) are correct but (R) is not the correct explanation of (A)
  3. C(A) is correct but (R) is not correct
  4. D(A) is not correct but (R) is correct

Correct answer

C. (A) is correct but (R) is not correct

Step-by-step solution

Assertion (A) is correct because the Pvu I restriction site is located within the ampicillin resistance gene ( amp^R ) of the pBR322 vector. Insertion of a foreign DNA at this site causes insertional inactivation of the amp^R gene, rendering the transformants susceptible to ampicillin. Reason (R) is incorrect because the 'rop' gene contains the restriction site for Pvu II, not Pvu I. The 'rop' gene codes for the proteins involved in the replication of the plasmid. Answer: (A) is correct but (R) is not correct

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