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NEETZoologyBiotechnology - Principles and Processes

Match List-I with List-II regarding the steps in recombinant DNA technology and their corresponding tools or techniques. List-I List-II A. Cutting of DNA at specific locations I. Restriction Endonuclease B. Isolation of desired DNA fragment II. Agarose gel electrophoresis C. Amplification of gene of interest III. Polymerase Chain Reaction D. Insertion of recombinant DNA into host IV. Transformation Choose the correct

Options

  1. AA-II, B-I, C-III, D-IV
  2. BA-I, B-II, C-IV, D-III
  3. CA-I, B-II, C-III, D-IV
  4. DA-IV, B-II, C-III, D-I

Correct answer

C. A-I, B-II, C-III, D-IV

Step-by-step solution

Cutting of DNA at specific locations is carried out by restriction endonucleases (A-I). After cutting, the DNA fragments are separated and the desired DNA fragment is isolated using agarose gel electrophoresis (B-II). The isolated gene of interest is then amplified using Polymerase Chain Reaction (PCR) (C-III). Finally, the recombinant DNA is introduced into a competent host cell through processes like transformation (D-IV). Therefore, the correct match is A-I, B-II, C-III, D-IV. Answer: A-I, B-II, C-III, D-IV

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