Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

While performing an experiment to isolate genetic material, a student adds a specific chemical to a purified mixture of biomolecules. This causes the purified DNA to precipitate out as fine threads in the suspension, which are then physically removed using a glass rod. Identify the chemical added and the specific term used for this extraction process.

Options

  1. AChilled ethanol and Elution
  2. BChilled ethanol and Spooling
  3. CRoom temperature ethanol and Spooling
  4. DChilled chloroform and Spooling

Correct answer

B. Chilled ethanol and Spooling

Step-by-step solution

In the final step of DNA isolation, purified DNA precipitates out after the addition of chilled ethanol. This precipitation can be seen as a collection of fine threads in the suspension. The physical process of removing these precipitated DNA threads from the suspension, typically using a glass rod, is known as spooling. Elution refers to the extraction of separated DNA fragments from an agarose gel piece, which is a different process. Room temperature ethanol or chilled chloroform will not effectively precipitate

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs