NEETZoologyBiotechnology - Principles and Processes
While performing an experiment to isolate genetic material, a student adds a specific chemical to a purified mixture of biomolecules. This causes the purified DNA to precipitate out as fine threads in the suspension, which are then physically removed using a glass rod. Identify the chemical added and the specific term used for this extraction process.
Options
- AChilled ethanol and Elution
- BChilled ethanol and Spooling
- CRoom temperature ethanol and Spooling
- DChilled chloroform and Spooling
Correct answer
B. Chilled ethanol and Spooling
Step-by-step solution
In the final step of DNA isolation, purified DNA precipitates out after the addition of chilled ethanol. This precipitation can be seen as a collection of fine threads in the suspension. The physical process of removing these precipitated DNA threads from the suspension, typically using a glass rod, is known as spooling. Elution refers to the extraction of separated DNA fragments from an agarose gel piece, which is a different process. Room temperature ethanol or chilled chloroform will not effectively precipitate