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Match List-I with List-II regarding the methods of gene transfer into host cells. List-I List-II (a) Biolistics (i) Direct injection of recombinant DNA into the nucleus of animal cells (b) Micro-injection (ii) Increases the efficiency with which DNA enters the bacterium through pores in its cell wall (c) Calcium ion treatment (iii) Bombardment of plant cells with high velocity micro-particles of gold or tungsten coat

Options

  1. A(a)-(i), (b)-(iii), (c)-(ii), (d)-(iv)
  2. B(a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
  3. C(a)-(iii), (b)-(i), (c)-(ii), (d)-(iv)
  4. D(a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)

Correct answer

C. (a)-(iii), (b)-(i), (c)-(ii), (d)-(iv)

Step-by-step solution

Biolistics (gene gun) involves bombarding plant cells with high-velocity micro-particles of gold or tungsten coated with DNA, making (a) match with (iii). Micro-injection involves directly injecting recombinant DNA into the nucleus of an animal cell, making (b) match with (i). Calcium ion (divalent cation) treatment increases the efficiency with which DNA enters a bacterium through pores in its cell wall, making (c) match with (ii). Disarmed retroviruses are used as vectors to deliver desirable genes into animal ce

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