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NEETZoologyBiotechnology - Principles and Processes

During the isolation of genetic material from a cell extract, it is necessary to remove other macromolecules to obtain purified DNA. Which of the following pairs of enzymes is specifically used to remove RNA and proteins, respectively, from the mixture?

Options

  1. ARibonuclease and Protease
  2. BDNase and Protease
  3. CRibonuclease and Lipase
  4. DCellulase and Protease

Correct answer

A. Ribonuclease and Protease

Step-by-step solution

During the isolation of DNA, the cell extract contains various macromolecules such as RNA, proteins, lipids, and polysaccharides. According to NCERT, RNA can be removed by treatment with ribonuclease, whereas proteins can be removed by treatment with protease. This ensures that only purified DNA remains for the subsequent precipitation step. Using DNase would degrade the DNA itself, which is the desired genetic material. Cellulase is used to break down plant cell walls, and lipase is used for lipids, not for the pr

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