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Match the restriction enzymes used to insert a foreign DNA into the pBR322 vector (List-I) with the resulting phenotype or status of the recombinant plasmid (List-II). List-I List-II (A) BamHI (I) Ampicillin sensitive and Tetracycline resistant (B) PvuI (II) Inactivation of the rop gene (C) EcoRI (III) Ampicillin resistant and Tetracycline sensitive (D) PvuII (IV) Both Ampicillin and Tetracycline resistant Choose the

Options

  1. A(A)-(I), (B)-(III), (C)-(II), (D)-(IV)
  2. B(A)-(III), (B)-(I), (C)-(II), (D)-(IV)
  3. C(A)-(I), (B)-(III), (C)-(IV), (D)-(II)
  4. D(A)-(III), (B)-(I), (C)-(IV), (D)-(II)

Correct answer

D. (A)-(III), (B)-(I), (C)-(IV), (D)-(II)

Step-by-step solution

The pBR322 vector contains two antibiotic resistance genes: ampicillin resistance and tetracycline resistance. Insertion of foreign DNA at the BamHI site inactivates the tetracycline resistance gene, resulting in a plasmid that is ampicillin resistant but tetracycline sensitive ((A)-(III)). Insertion at the PvuI site inactivates the ampicillin resistance gene, making the plasmid ampicillin sensitive but tetracycline resistant ((B)-(I)). The EcoRI site is located outside both antibiotic resistance genes, so insertio

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