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Match List I with List II regarding the process of gel electrophoresis. List I List II A. Agarose I. Extraction of separated DNA from gel piece B. Ethidium bromide II. Matrix used for separation of DNA fragments C. UV radiation III. Stain used to visualize DNA fragments D. Elution IV. Required to observe bright orange coloured bands of DNA Choose the correct answer from the options given below:

Options

  1. AA - II, B - III, C - IV, D - I
  2. BA - II, B - IV, C - III, D - I
  3. CA - I, B - III, C - IV, D - II
  4. DA - II, B - I, C - IV, D - III

Correct answer

A. A - II, B - III, C - IV, D - I

Step-by-step solution

In gel electrophoresis, agarose is a natural polymer extracted from seaweeds that acts as a sieving matrix for the separation of DNA fragments (A matches II). Ethidium bromide is the specific chemical stain used to make the separated DNA fragments visible (B matches III). UV radiation is the specific electromagnetic radiation required to illuminate the ethidium bromide-stained DNA, causing it to fluoresce as bright orange bands (C matches IV). Elution is the procedural step where the separated bands of DNA are cut

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