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Match List I with List II regarding the methods of introducing recombinant DNA into host cells. List I List II A. Direct method for plant cells I. Microinjection B. Direct method for animal cells II. Biolistics C. Disarmed pathogen vector for plant cells III. Disarmed retroviruses D. Disarmed pathogen vector for animal cells IV. Agrobacterium tumefaciens Choose the correct answer from the options given below:

Options

  1. AA-II, B-I, C-III, D-IV
  2. BA-I, B-II, C-IV, D-III
  3. CA-II, B-I, C-IV, D-III
  4. DA-IV, B-III, C-II, D-I

Correct answer

C. A-II, B-I, C-IV, D-III

Step-by-step solution

Biolistics (gene gun) is a direct method used to introduce recombinant DNA into plant cells (A-II). Microinjection is a direct method where recombinant DNA is directly injected into the nucleus of an animal cell (B-I). Agrobacterium tumefaciens is a pathogen of dicot plants that has been disarmed and used as a vector to deliver genes into plant cells (C-IV). Retroviruses, which normally transform animal cells into cancerous cells, are disarmed and used as vectors to deliver desirable genes into animal cells (D-III)

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