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Match List-I with List-II regarding biotechnology techniques and their applications. List-I List-II (A) PCR (I) Antigen-antibody interaction (B) ELISA (II) Separation and purification of protein product (C) Autoradiography (III) Amplification of nucleic acids (D) Downstream processing (IV) Detection of mutated gene using a radioactive probe Choose the correct answer from the options given below:

Options

  1. A(A)-(II), (B)-(I), (C)-(IV), (D)-(III)
  2. B(A)-(III), (B)-(I), (C)-(IV), (D)-(II)
  3. C(A)-(III), (B)-(IV), (C)-(I), (D)-(II)
  4. D(A)-(IV), (B)-(I), (C)-(III), (D)-(II)

Correct answer

B. (A)-(III), (B)-(I), (C)-(IV), (D)-(II)

Step-by-step solution

Let us match each technique with its correct principle or application: (A) PCR (Polymerase Chain Reaction) is used for the in vitro amplification of nucleic acids (DNA). (B) ELISA (Enzyme-Linked Immunosorbent Assay) is a diagnostic test based on the principle of antigen-antibody interaction. (C) Autoradiography is used to detect mutated genes. A single-stranded DNA or RNA tagged with a radioactive molecule (probe) hybridises to its complementary DNA, and the clone having the mutated gene will not appear on the phot

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