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Match List-I with List-II regarding the enzymes used for isolating genetic material. List-I (Target Cell) List-II (Enzyme) A. Bacterial cell I. Cellulase B. Plant cell II. Chitinase C. Fungal cell III. Lysozyme IV. Ribonuclease Choose the correct answer from the options given below:

Options

  1. AA-II, B-I, C-III
  2. BA-III, B-II, C-I
  3. CA-I, B-IV, C-II
  4. DA-III, B-I, C-II

Correct answer

D. A-III, B-I, C-II

Step-by-step solution

In recombinant DNA technology, the isolation of genetic material requires breaking open the cells to release DNA. Bacterial cells have a cell wall made of peptidoglycan, which is degraded by the enzyme lysozyme (A-III). Plant cells have a cellulosic cell wall, which is degraded by the enzyme cellulase (B-I). Fungal cells have a cell wall made of chitin, which is degraded by the enzyme chitinase (C-II). Ribonuclease is used later in the process to remove RNA, not to break open the cell wall. Therefore, the correct m

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