Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

A recombinant pBR322 plasmid is constructed by inserting a foreign DNA fragment at the Pst I restriction site. If this recombinant plasmid is successfully transformed into E. coli cells, what will be the growth characteristics of these transformants on antibiotic-containing media?

Options

  1. AThey will grow on an ampicillin-containing medium but not on a tetracycline-containing medium.
  2. BThey will grow on a tetracycline-containing medium but not on an ampicillin-containing medium.
  3. CThey will fail to grow on both ampicillin and tetracycline-containing media.
  4. DThey will grow on both ampicillin and tetracycline-containing media.

Correct answer

B. They will grow on a tetracycline-containing medium but not on an ampicillin-containing medium.

Step-by-step solution

In the pBR322 cloning vector, the restriction site for Pst I is located within the amp^R (ampicillin resistance) gene. When a foreign DNA fragment is inserted at the Pst I site, it causes insertional inactivation of the amp^R gene. As a result, the recombinant plasmid loses ampicillin resistance but retains tetracycline resistance (since the tet^R gene remains intact). Therefore, the transformants containing the recombinant plasmid will be able to grow on a tetracycline-containing medium but will fail to grow on an

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs