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Which of the following methods is specifically suited for introducing recombinant DNA into animal cells?

Options

  1. ABiolistics
  2. BMicroinjection
  3. CHeat shock treatment
  4. DAgrobacterium tumefaciens

Correct answer

B. Microinjection

Step-by-step solution

In microinjection, recombinant DNA is directly injected into the nucleus of an animal cell. Biolistics (or gene gun) is a method suitable for plant cells, where cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA. Heat shock treatment is used to make bacterial cells competent to take up plasmids. Agrobacterium tumefaciens is a pathogen of several dicot plants and is used as a vector to introduce DNA into plant cells, not animal cells. Answer: Microinjection

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