NEETZoologyBiotechnology - Principles and Processes
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In the Polymerase Chain Reaction (PCR), standard human DNA polymerase is specifically chosen over Taq polymerase for the extension step. Reason (R): Taq polymerase is a thermostable enzyme that remains catalytically active during the high-temperature denaturation step of PCR. In the light of the ab
Options
- ABoth (A) and (R) are true and (R) is the correct explanation of (A)
- BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
- C(A) is true but (R) is false
- D(A) is false but (R) is true
Correct answer
D. (A) is false but (R) is true
Step-by-step solution
The Assertion (A) is false. In the Polymerase Chain Reaction (PCR), standard human DNA polymerase is not used because it would be permanently denatured by the high temperatures required to separate the DNA strands. Instead, Taq polymerase is specifically chosen for the extension step. The Reason (R) is true. Taq polymerase is isolated from the thermophilic bacterium Thermus aquaticus. It is a thermostable enzyme that remains catalytically active during the high-temperature denaturation step (approximately 94^ C ) o