NEETZoologyBiotechnology - Principles and Processes
Read the following statements regarding agarose gel electrophoresis: (A) Smaller DNA fragments move farther away from the well than larger fragments. (B) DNA fragments can be visualised directly under visible light after staining with ethidium bromide. (C) The extraction of separated DNA bands from the agarose gel is known as elution. (D) The agarose matrix provides a sieving effect for the DNA molecules. How many of
Options
- AFour
- BTwo
- CThree
- DOne
Correct answer
C. Three
Step-by-step solution
Statement (A) is correct: DNA fragments separate according to their size, and smaller fragments move farther through the gel. Statement (B) is incorrect: DNA fragments stained with ethidium bromide can only be visualised under UV light, not visible light. Statement (C) is correct: The process of cutting out and extracting the separated DNA bands from the agarose gel is called elution. Statement (D) is correct: The agarose gel acts as a matrix that provides a sieving effect to separate DNA molecules based on size. T